2WHK
Structure of Bacillus subtilis mannanase man26
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 31 |
Unit cell lengths | 67.498, 67.498, 72.966 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.480 - 1.700 |
R-factor | 0.172 |
Rwork | 0.170 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gw1 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.099 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.050 | 0.120 |
Number of reflections | 40383 | |
<I/σ(I)> | 44 | 7.1 |
Completeness [%] | 100.0 | 99 |
Redundancy | 7 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 35% PEG8K, 0.2M NA ACETATE, 0.1M TRIS PH 7 TO 8 |