2WGB
Crystal structure of the TetR-like transcriptional regulator LfrR from Mycobacterium smegmatis
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-04 |
Detector | MARRESEARCH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 86.646, 86.646, 96.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.323 - 2.000 |
R-factor | 0.1874 |
Rwork | 0.185 |
R-free | 0.22800 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.007 |
RMSD bond angle | 0.825 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | autoSHARP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.400 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.060 | 0.520 |
Number of reflections | 25569 | |
<I/σ(I)> | 31.6 | 5.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.5 | 14.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 10% PEG8000, 0.5 M LISO4, pH 8.0 |