2WG8
Structure of Oryza Sativa (Rice) PLA2, orthorhombic crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 169.094, 41.491, 53.124 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
R-factor | 0.19271 |
Rwork | 0.190 |
R-free | 0.24505 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | P62 CRYSTAL FORM |
RMSD bond length | 0.009 |
RMSD bond angle | 1.195 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.010 | 0.330 |
Number of reflections | 15584 | |
<I/σ(I)> | 17.4 | 5.4 |
Completeness [%] | 94.7 | 93.2 |
Redundancy | 4.8 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | VAPOUR DIFFUSION, 20 DEGREES. WELL CONTAINED 0.75ML OF SOLN A (16-18% PEG3350, 0.1M BISTRISPROPANE PH 6.5, 0.2M POTASSIUM THIOCYANATE) AND 0.25ML OF SOLN B (0.1M SODIUM ACETATE PH 4.6, 2M SODIUM CHLORIDE) |