2WFX
Crystal structure of the complex between human hedgehog-interacting protein HIP and Sonic Hedgehog in the presence of calcium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 89.150, 89.150, 171.429 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.774 - 3.200 |
R-factor | 0.2448 |
Rwork | 0.240 |
R-free | 0.30030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1VHH AND 2WFT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.524 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.300 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.190 | 0.730 |
Number of reflections | 13529 | |
<I/σ(I)> | 10.6 | 2.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.5 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M SODIUM ACETATE, PH 4.6 0.5 M POTASSIUM THIOCYANATE 5 MM CACL |