2WF8
Structure of Beta-Phosphoglucomutase inhibited with Glucose-6- phosphate, Glucose-1-phosphate and Beryllium trifluoride
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.300, 54.300, 104.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.200 |
R-factor | 0.163 |
Rwork | 0.162 |
R-free | 0.19100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wf5 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.627 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.260 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.040 | 0.380 |
Number of reflections | 61544 | |
<I/σ(I)> | 18.4 | 2.7 |
Completeness [%] | 92.3 | 66.9 |
Redundancy | 4.4 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 28-29 % PEG 4000 AND 200 MM NA ACETATE, pH 7.5 |