2WEP

Yeast Hsp90 N-terminal domain LI-IV mutant with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 43 2 2
Unit cell lengths	74.915, 74.915, 110.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.060 - 2.000
R-factor	0.21246
Rwork	0.210
R-free	0.26305
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	IN-HOUSE HSP90 N-TERMINAL MODEL.
RMSD bond length	0.020
RMSD bond angle	1.769
Data reduction software	iMOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.4.0066)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.060	0.500
Number of reflections	21977
<I/σ(I)>	8.2	3.2
Completeness [%]	99.9	100
Redundancy	5.3	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		pH 7