2WE3
EBV dUTPase inactive mutant deleted of motif V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-10 |
| Detector | ADSC CCD |
| Spacegroup name | I 4 |
| Unit cell lengths | 103.238, 103.238, 47.697 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.640 - 2.000 |
| R-factor | 0.215 |
| Rwork | 0.212 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bsy |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.719 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0038) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.900 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.430 |
| Number of reflections | 17157 | |
| <I/σ(I)> | 7.2 | 1.7 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS PH 8.5, 20 % PEG 3350, 200 MM LISO4, AND 10 MM DUTP |
