2WE1
EBV dUTPase mutant Asp131Asn with bound dUMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-10 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.624, 56.859, 81.073 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.570 - 1.800 |
R-factor | 0.1982 |
Rwork | 0.196 |
R-free | 0.24740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bsy |
RMSD bond length | 0.016 |
RMSD bond angle | 1.632 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.570 | 1.900 |
High resolution limit [Å] | 1.380 | 1.800 |
Rmerge | 0.080 | 0.350 |
Number of reflections | 24052 | |
<I/σ(I)> | 6.56 | 2.16 |
Completeness [%] | 98.8 | 99.9 |
Redundancy | 3.45 | 3.43 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 0.3 M AMMONIUM SULFATE, 25 % PEG 3350, 0.1 M HEPES PH 7, 10 MM DUTP |