2WE0
EBV dUTPase mutant Cys4Ser
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.048, 56.783, 81.374 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.570 - 2.010 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bsy |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.921 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.520 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.340 |
| Number of reflections | 23739 | |
| <I/σ(I)> | 5 | 2.2 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 6.87 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 150 MM MALIC ACID PH 7.5, 25 % PEG 3350 AND 10 MM DUTP. |
