2WDP
Crystal Structure of Ligand Free Human Caspase-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.146, 90.441, 86.397 |
| Unit cell angles | 90.00, 92.77, 90.00 |
Refinement procedure
| Resolution | 63.072 - 1.950 |
| R-factor | 0.2262 |
| Rwork | 0.224 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k86 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.805 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.250 | |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.080 | 0.490 |
| Number of reflections | 69153 | |
| <I/σ(I)> | 13.6 | 3 |
| Completeness [%] | 97.7 | 96.3 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 1.5 M AMMONIUM CHLORIDE, 0.1 M SODIUM ACETATE AT PH 4.6 |
