2WD6
Crystal structure of the Variable Domain of the Streptococcus gordonii Surface Protein SspB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 110.170, 110.170, 121.490 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 95.350 - 2.300 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.20900 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.634 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.750 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.520 |
| Number of reflections | 37172 | |
| <I/σ(I)> | 24.4 | 4.4 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 10.99 | 10.42 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M KBR 25% PEG3350 |
