2WCW
1.6A resolution structure of Archaeoglobus fulgidus Hjc, a Holliday junction resolvase from an archaeal hyperthermophile
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.510, 75.480, 60.920 |
| Unit cell angles | 90.00, 115.34, 90.00 |
Refinement procedure
| Resolution | 29.180 - 1.580 |
| R-factor | 0.156 |
| Rwork | 0.154 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WILD-TYPE CRYSTAL FORM |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.128 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.620 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.050 | 0.660 |
| Number of reflections | 60375 | |
| <I/σ(I)> | 15.48 | 2.06 |
| Completeness [%] | 93.5 | 79.6 |
| Redundancy | 4.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 4.5 M AMMONIUM ACETATE 0.1 M TRIS-HCL PH 8.5 |
