2WCT
human SARS coronavirus unique domain (triclinic form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-27 |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 68.680, 75.520, 80.540 |
Unit cell angles | 77.16, 75.61, 74.48 |
Refinement procedure
Resolution | 33.330 - 2.790 |
R-factor | 0.2242 |
Rwork | 0.223 |
R-free | 0.23990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jze |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (2.7.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.330 | 2.960 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.080 | 0.250 |
Number of reflections | 34003 | |
<I/σ(I)> | 8.8 | 2 |
Completeness [%] | 93.1 | 76.6 |
Redundancy | 3 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 25% PEG MME 5000, 0.1 M MES PH6.5, 0.2 M AMMONIUM SULFATE |