2WCM
Structure of BMori GOBP2 (General Odorant Binding Protein 2) with (10E)-hexadecen-12-yn-1-ol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-30 |
Detector | ADSC CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 33.209, 33.209, 189.363 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 62.500 - 1.500 |
R-factor | 0.199 |
Rwork | 0.197 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wc5 |
RMSD bond length | 0.023 |
RMSD bond angle | 1.980 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.200 | 1.540 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.110 | 0.380 |
Number of reflections | 17328 | |
<I/σ(I)> | 14.8 | 3.8 |
Completeness [%] | 92.9 | 95.4 |
Redundancy | 6.4 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 30% PEG 4000, 200MM MGCL2, 100MM TRIS PH 8.5 |