2WC7

Crystal structure of Nostoc Punctiforme Debranching Enzyme(NPDE)(Acarbose soaked)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	95
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 61 2 2
Unit cell lengths	86.968, 86.968, 262.393
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.540 - 2.370
R-factor	0.215
Rwork	0.215
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j0h
RMSD bond length	0.006
RMSD bond angle	1.200
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.450
High resolution limit [Å]	2.370	2.370
Rmerge	0.090	0.270
Number of reflections	24597
<I/σ(I)>	10.8	5.09
Completeness [%]	99.0	98.8
Redundancy	12.8	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		20%(W/V)PEG 8000,0.2M TRIC-CL, 0.1M MGCL2, pH 7.5