2WC5
Structure of BMori GOBP2 (General Odorant Binding Protein 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-13 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 33.160, 33.160, 189.672 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.600 - 1.900 |
| R-factor | 0.172 |
| Rwork | 0.168 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dqe |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.835 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.600 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.230 |
| Number of reflections | 9783 | |
| <I/σ(I)> | 11.1 | 6.5 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 4.7 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 30% PEG 4000, 200MM MGCL2, 100MM TRIS PH 8.5 |
