2WB6
Crystal structure of AFV1-102, a protein from the Acidianus Filamentous Virus 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 62.890, 62.890, 110.837 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 54.470 - 1.950 |
R-factor | 0.21619 |
Rwork | 0.213 |
R-free | 0.27431 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.011 |
RMSD bond angle | 1.313 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.470 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.110 | 0.590 |
Number of reflections | 10006 | |
<I/σ(I)> | 4.4 | 0.8 |
Completeness [%] | 99.5 | 100 |
Redundancy | 34.7 | 38 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 1.5-2M AMMONIUM SULFATE, 0.1M HEPES PH 7.5, 2% PEG400 |