2WB0
2.1 resolution structure of the C-terminal domain of the human adenovirus 5 ssDNA binding protein
Replaces: 1ADTExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.290, 76.660, 64.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.950 - 1.950 |
R-factor | 0.187 |
Rwork | 0.184 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ADT |
RMSD bond length | 0.026 |
RMSD bond angle | 2.250 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.050 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.060 | 0.320 |
Number of reflections | 29937 | |
<I/σ(I)> | 21.3 | 4.5 |
Completeness [%] | 98.6 | 99.3 |
Redundancy | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.8 | pH 5.8 |