2WAZ
N512P mutant of the DNA binding domain of the Adenovirus 5 ssDNA binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.320, 75.930, 64.635 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.870 - 2.300 |
| R-factor | 0.171 |
| Rwork | 0.167 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ADT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.934 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.450 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.110 |
| Number of reflections | 16993 | |
| <I/σ(I)> | 17.6 | 8 |
| Completeness [%] | 88.6 | 97.4 |
| Redundancy | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.0M NANO3 WITH SODIUM ACETATE BUFFER AT PH5.8 |
