2WAU
Structure of DBL6 epsilon domain from VAR2CSA
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-08 |
Detector | ADSC CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 62.943, 62.943, 334.269 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 83.620 - 3.000 |
R-factor | 0.29 |
Rwork | 0.289 |
R-free | 0.32500 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.006 |
RMSD bond angle | 0.893 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 111.100 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.100 | 0.600 |
Number of reflections | 14481 | |
<I/σ(I)> | 14.2 | 2.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.8 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | pH 8 | ||
1 | 8 | pH 8 |