2WA9
Structural basis of N-end rule substrate recognition in Escherichia coli by the ClpAP adaptor protein ClpS - Trp peptide structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 90 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 171.910, 155.870, 71.230 |
Unit cell angles | 90.00, 114.64, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.900 |
R-factor | 0.237 |
Rwork | 0.236 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.570 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.020 | 0.990 |
Number of reflections | 19203 | |
<I/σ(I)> | 11 | 2 |
Completeness [%] | 99.3 | 96.3 |
Redundancy | 7.5 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | pH 8.5 |