2WA8
Structural basis of N-end rule substrate recognition in Escherichia coli by the ClpAP adaptor protein ClpS - The Phe peptide structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 90 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.223, 58.405, 56.413 |
Unit cell angles | 90.00, 101.89, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.150 |
R-factor | 0.228 |
Rwork | 0.225 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.542 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.270 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.010 | 0.450 |
Number of reflections | 10728 | |
<I/σ(I)> | 6.6 | 2.3 |
Completeness [%] | 94.0 | 90.5 |
Redundancy | 2.8 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | pH 8.5 |