2W9Y
The structure of the lipid binding protein Ce-FAR-7 from Caenorhabditis elegans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.850, 41.600, 101.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.900 - 1.800 |
| R-factor | 0.174 |
| Rwork | 0.171 |
| R-free | 0.25100 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.676 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.090 | 0.590 |
| Number of reflections | 13757 | |
| <I/σ(I)> | 10.13 | 2.19 |
| Completeness [%] | 97.7 | 94.6 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20MM TRIS PH 7.8-8.5, 2.1-2.9M AMMONIUM SULFATE | ||
| 1 | 7.5 | 20MM TRIS PH 7.8-8.5, 2.1-2.9M AMMONIUM SULFATE |
