2W9N
crystal structure of linear di-ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 105.572, 105.572, 105.572 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.250 |
| R-factor | 0.26 |
| Rwork | 0.259 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.927 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.740 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.110 | 0.540 |
| Number of reflections | 9985 | |
| <I/σ(I)> | 27.4 | 2 |
| Completeness [%] | 99.1 | 96.9 |
| Redundancy | 17.1 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 22% PEG 3350 AND 200 MM ZNOAC, pH 7 |
