2W9M
Structure of family X DNA polymerase from Deinococcus radiodurans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.930, 138.250, 67.750 |
Unit cell angles | 90.00, 92.25, 90.00 |
Refinement procedure
Resolution | 45.320 - 2.460 |
R-factor | 0.206 |
Rwork | 0.203 |
R-free | 0.24900 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.004 |
RMSD bond angle | 0.813 |
Phasing software | PHENIX (HYSS) |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.500 |
High resolution limit [Å] | 2.460 | 2.460 |
Rmerge | 0.070 | 0.350 |
Number of reflections | 155376 | |
<I/σ(I)> | 13 | 3 |
Completeness [%] | 95.4 | 77.7 |
Redundancy | 4.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |