2W9J

The crystal structure of SRP14 from the Schizosaccharomyces pombe signal recognition particle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-19
Detector	ADSC CCD
Spacegroup name	P 32 2 1
Unit cell lengths	57.040, 57.040, 86.370
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.620 - 2.600
R-factor	0.24
Rwork	0.238
R-free	0.28100
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.010
RMSD bond angle	1.243
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.4.0065)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	5.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.060	0.410
Number of reflections	5174
<I/σ(I)>	16	5
Completeness [%]	97.4	95.5
Redundancy	5.8	6.17

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				50MM CACODYLIC ACID, PH 6.5, 22% POLY-ETHYLENE GLYCOL 4000, 50MM SODIUM ACETATE
1				50MM CACODYLIC ACID, PH 6.5, 22% POLY-ETHYLENE GLYCOL 4000, 50MM SODIUM ACETATE