2W94
Native structure of the Discoidin I from Dictyostelium discoideum at 1.8 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-20 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.530, 274.371, 85.928 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.600 - 1.800 |
| R-factor | 0.16679 |
| Rwork | 0.165 |
| R-free | 0.20593 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vm9 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.496 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.600 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.230 |
| Number of reflections | 29532 | |
| <I/σ(I)> | 5.9 | 2 |
| Completeness [%] | 94.8 | 94.8 |
| Redundancy | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1.5 M LISO4 100 MM HEPES 7.5 |
