2W8C
Plastocyanin variant with N-terminal Methionine - closed structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | H 3 |
| Unit cell lengths | 55.740, 55.740, 197.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.940 - 1.800 |
| R-factor | 0.176 |
| Rwork | 0.173 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2W88 CHAIN A |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.274 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.900 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.290 |
| Number of reflections | 20932 | |
| <I/σ(I)> | 8.6 | 2.7 |
| Completeness [%] | 99.1 | 93.7 |
| Redundancy | 5.4 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5% PEG 8K, 0.1M ZINC ACETATE, 0.2 M SODIUM ACETATE |
