2W8C
Plastocyanin variant with N-terminal Methionine - closed structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | H 3 |
Unit cell lengths | 55.740, 55.740, 197.850 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 65.940 - 1.800 |
R-factor | 0.176 |
Rwork | 0.173 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2W88 CHAIN A |
RMSD bond length | 0.011 |
RMSD bond angle | 1.274 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.900 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.060 | 0.290 |
Number of reflections | 20932 | |
<I/σ(I)> | 8.6 | 2.7 |
Completeness [%] | 99.1 | 93.7 |
Redundancy | 5.4 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5% PEG 8K, 0.1M ZINC ACETATE, 0.2 M SODIUM ACETATE |