2W89
Crystal structure of the E.coli tRNAArg aminoacyl stem issoacceptor RR-1660 at 2.0 Angstroem resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 203 |
Detector technology | CCD |
Collection date | 2008-07-31 |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 26.368, 28.984, 29.134 |
Unit cell angles | 105.89, 98.76, 97.85 |
Refinement procedure
Resolution | 20.920 - 2.000 |
R-factor | 0.214 |
Rwork | 0.212 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vuq |
RMSD bond length | 0.019 |
RMSD bond angle | 2.567 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.040 | 0.160 |
Number of reflections | 7118 | |
<I/σ(I)> | 22.2 | 1.9 |
Completeness [%] | 95.0 | 93.2 |
Redundancy | 1.8 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 294 | 10 MM MAGNESIUM CHLORIDE, 50 MM MES, PH 5.6, 2 M LITHIUM SULFATE, 294K, CRYOPROTECTANT 20 % (V/V) GLYCEROL |