2W88
Plastocyanin variant with N-terminal Methionine - open structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector | RIGAKU IMAGING PLATE |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 127.700, 127.700, 94.990 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 110.430 - 2.890 |
R-factor | 0.19578 |
Rwork | 0.194 |
R-free | 0.22285 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cvb |
RMSD bond length | 0.011 |
RMSD bond angle | 1.351 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.780 | 3.050 |
High resolution limit [Å] | 2.890 | 2.890 |
Rmerge | 0.170 | 0.580 |
Number of reflections | 19857 | |
<I/σ(I)> | 4.3 | 1.3 |
Completeness [%] | 99.8 | 98.4 |
Redundancy | 7.5 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M ZINC ACETATE, 0.4 M SODIUM ACETATE |