2W7N
Crystal Structure of KorA Bound to Operator DNA: Insight into Repressor Cooperation in RP4 Gene Regulation
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-27 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.830, 115.450, 49.880 |
Unit cell angles | 90.00, 114.00, 90.00 |
Refinement procedure
Resolution | 57.740 - 1.850 |
R-factor | 0.172 |
Rwork | 0.170 |
R-free | 0.20100 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.006 |
RMSD bond angle | 1.522 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | XDS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.080 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.030 | 0.070 |
Number of reflections | 31085 | |
<I/σ(I)> | 42.5 | 17.4 |
Completeness [%] | 93.3 | 70.1 |
Redundancy | 7.4 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.5M (NH4)2SO4, 0.1M C6H5NA3O7 2H2O (PH 5.6), 1.0M LI2SO4 H2O, VAPOR DIFFUSION, HANGING DROP, 291 K |