2W75
Structures of P. aeruginosa FpvA bound to heterologous pyoverdines: Apo-FpvA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.794, 129.526, 140.952 |
| Unit cell angles | 90.00, 130.57, 90.00 |
Refinement procedure
| Resolution | 107.210 - 2.900 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o5p |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.939 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.4.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.100 | 0.260 |
| Number of reflections | 53997 | |
| <I/σ(I)> | 1.9 | 1.9 |
| Completeness [%] | 92.3 | 93.3 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.3M NAH2PO4, 0.1M MES, PH 6.5 |
