2W6W
Crystal structure of recombinant Sperm Whale Myoglobin under 1atm of Xenon
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-04-20 |
Detector | RIGAKU-MSC |
Spacegroup name | P 6 |
Unit cell lengths | 90.626, 90.626, 45.505 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.990 |
R-factor | 0.189 |
Rwork | 0.188 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1naz |
RMSD bond length | 0.006 |
RMSD bond angle | 0.875 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.680 | 2.010 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.050 | 0.120 |
Number of reflections | 15044 | |
<I/σ(I)> | 7 | 3.1 |
Completeness [%] | 98.1 | 97.3 |
Redundancy | 3.2 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 2.6M (NH4)2SO4, 0.1M TRIS/HCL, PH8.5 |