2W62
Saccharomyces cerevisiae Gas2p in complex with laminaripentaose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector | RIGAKU |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.039, 70.841, 149.149 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.000 - 1.850 |
R-factor | 0.184 |
Rwork | 0.183 |
R-free | 0.21000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | DERIVATIVE MODEL NOT DEPOSITED |
RMSD bond length | 0.012 |
RMSD bond angle | 1.309 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.040 | 0.290 |
Number of reflections | 45921 | |
<I/σ(I)> | 23.3 | 5.5 |
Completeness [%] | 99.7 | 100 |
Redundancy | 5.3 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 100 MM SODIUM ACETATE PH 4.5; 5% ACETONE; 20% 1,4-BUTANEDIOL |