2W5R
Structure-based mechanism of lipoteichoic acid synthesis by Staphylococcus aureus LtaS.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.817, 57.133, 160.631 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.100 - 1.700 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.20500 |
| Structure solution method | OTHER |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.280 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 41.100 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.080 |
| Number of reflections | 46744 |
| <I/σ(I)> | 6.4 |
| Completeness [%] | 99.9 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 30% PEG4000, 100MM SODIUM CITRATE, PH5.6, 200MM AMMONIUM ACETATE, 30MG/ML GLYCEROL-PHOSPHATE |
