2W5J
Structure of the c14-rotor ring of the proton translocating chloroplast ATP synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 128.601, 89.996, 124.893 |
Unit cell angles | 90.00, 104.70, 90.00 |
Refinement procedure
Resolution | 19.750 - 3.800 |
R-factor | 0.318 |
Rwork | 0.317 |
R-free | 0.33500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yce |
RMSD bond length | 0.013 |
RMSD bond angle | 1.284 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 4.010 | 4.010 |
High resolution limit [Å] | 3.800 | 3.800 |
Rmeas | 0.085 | 0.248 |
Number of reflections | 12655 | 1852 |
<I/σ(I)> | 4.1 | 3.3 |
Completeness [%] | 92.2 | 92.2 |
Redundancy | 2.6 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |