2W5H
Human Nek2 kinase Apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.739, 56.962, 80.612 |
| Unit cell angles | 90.00, 133.36, 90.00 |
Refinement procedure
| Resolution | 58.610 - 2.330 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.25400 |
| Structure solution method | OTHER |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.964 |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 58.600 |
| High resolution limit [Å] | 2.330 |
| Rmerge | 0.110 |
| Number of reflections | 12969 |
| <I/σ(I)> | 6.1 |
| Completeness [%] | 91.3 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 50 MM TRIS, PH 8.5 2-10% PEG8000 |
