2W56
Structure of the hypothetical protein VC0508 from Vibrio cholerae VSP- II pathogenicity island
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | RIGAKU SATURN 944 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.102, 70.596, 51.382 |
Unit cell angles | 90.00, 110.64, 90.00 |
Refinement procedure
Resolution | 48.060 - 1.900 |
R-factor | 0.227 |
Rwork | 0.224 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v1l |
RMSD bond length | 0.010 |
RMSD bond angle | 1.339 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.030 | 0.030 |
Number of reflections | 21026 | |
<I/σ(I)> | 62 | 5.6 |
Completeness [%] | 97.0 | 96 |
Redundancy | 6.5 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | PH 5.5 |