2W4C
Human common-type acylphosphatase variant, A99
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-30 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.604, 57.861, 59.095 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.340 - 1.520 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vh7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.353 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.310 | 1.520 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.040 | 0.180 |
| Number of reflections | 13607 | |
| <I/σ(I)> | 30.7 | 9.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 16MG/ML K99A IN 20MMTRIS, 0.2MNACL, PH7.5 K99A WAS CRYSTALLIZED IN 0.1M BIS-TRIS, 0.1M NAOAC, PH6.0 WITH 25% PEG3350 AS CYROPROTECTANT |
