2W45
Epstein-Barr virus alkaline nuclease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-17 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 87.510, 63.787, 114.131 |
Unit cell angles | 90.00, 93.59, 90.00 |
Refinement procedure
Resolution | 71.430 - 3.000 |
R-factor | 0.19691 |
Rwork | 0.192 |
R-free | 0.28179 |
Structure solution method | SIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.019 |
RMSD bond angle | 2.085 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0068) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.800 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.140 | 0.440 |
Number of reflections | 24158 | |
<I/σ(I)> | 3.59 | 1.58 |
Completeness [%] | 94.9 | 96.48 |
Redundancy | 5.77 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | PROTEIN AT 4.5 MG/ML IN 250 MM NACL, 20MM TRIS PH 7.5, RESERVOIR:10 MM DTT, 10 MM MGCL2, 0.1M HEPES PH 7.0, 1.5% PEG 400 |