2W3E
Oxidized structure of the first GAF domain of Mycobacterium tuberculosis DosS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.000, 86.865, 100.642 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.560 - 1.600 |
| R-factor | 0.20132 |
| Rwork | 0.200 |
| R-free | 0.23404 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.427 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.070 |
| Number of reflections | 41345 |
| <I/σ(I)> | 26 |
| Completeness [%] | 96.8 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG4000 AND 0.2 M CALCIUM CHLORIDE IN TRIS-HCL, PH 7.0 |
