2W2R

Structure of the vesicular stomatitis virus matrix protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-17
Detector	ADSC CCD
Spacegroup name	I 4
Unit cell lengths	86.260, 86.260, 70.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.830 - 1.830
R-factor	0.158
Rwork	0.157
R-free	0.17900
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.011
RMSD bond angle	1.249
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	autoSHARP
Refinement software	REFMAC (5.4.0077)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.160	1.880
High resolution limit [Å]	1.830	1.830
Rmerge	0.120	0.840
Number of reflections	22813
<I/σ(I)>	10.7	2
Completeness [%]	99.8	99.6
Redundancy	7.3	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6		SITTING DROPS CONTAINING 100 NL 1.2 MG/ML PROTEIN AND 100 NL OF RESERVOIR SOLUTION (20% V/V ISOPROPANOL, 20% W/V PEG 4000 AND 0.1 M SODIUM CITRATE (PH 5.6)) WERE EQUILIBRATED AGAINST 95 UL RESERVIOURS AT 20.5C. CRYSTALS WERE CRYOPROTECTED BY A QUICK SWEEP THROUGH RESERVOIR SUPPLEMENTED WITH 20% V/V GLYCEROL.