2W2G
Human SARS coronavirus unique domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.360, 68.550, 94.210 |
| Unit cell angles | 90.00, 99.17, 90.00 |
Refinement procedure
| Resolution | 27.810 - 2.220 |
| R-factor | 0.214 |
| Rwork | 0.211 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jze |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | BUSTER-TNT (2.7.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.250 | 2.340 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.055 | 0.373 |
| Number of reflections | 26598 | |
| <I/σ(I)> | 16.9 | 2 |
| Completeness [%] | 92.1 | 61 |
| Redundancy | 6.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 20% PEGMME 5000,0.2M AMMONIUM SULFATE, 0.1M MES PH6.5 |
