2W29
Gly102Thr mutant of Rv3291c
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 83.000, 185.700, 119.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 4.100 |
Rwork | 0.388 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ivm |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 4.320 |
High resolution limit [Å] | 4.100 | 4.100 |
Rmerge | 0.140 | 0.410 |
Number of reflections | 23928 | |
<I/σ(I)> | 8.6 | 2.2 |
Completeness [%] | 94.4 | 83.7 |
Redundancy | 3.2 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | pH 6.0 |