2W25
Crystal structure of Glu104Ala mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 100.650, 100.650, 99.306 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.250 - 2.150 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ivm |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.269 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.320 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.100 | 0.800 |
| Number of reflections | 25538 | |
| <I/σ(I)> | 12.9 | 2.2 |
| Completeness [%] | 91.3 | 94.7 |
| Redundancy | 5.6 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | pH 6.0, reservoir: 100 mM 4-morpholineethanesulfonic acid (Mes), pH 6.0, 0.75 M lithium acetate (LiAc) |
