2W20
Structure of the catalytic domain of the native NanA sialidase from Streptococcus pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.200, 96.600, 218.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.230 - 1.490 |
R-factor | 0.201 |
Rwork | 0.200 |
R-free | 0.22500 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.000 |
RMSD bond angle | 1.220 |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.490 |
Rmerge | 0.070 |
Number of reflections | 159417 |
<I/σ(I)> | 16 |
Completeness [%] | 97.0 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 25% PEG4K, 0.1 M MES PH6.0 |