2W1Z
ROP2 from Toxoplasma gondii: A virulence factor with a protein- kinase fold and no enzymatic activity.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-07 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 187.609, 51.348, 125.582 |
| Unit cell angles | 90.00, 128.65, 90.00 |
Refinement procedure
| Resolution | 73.320 - 1.970 |
| R-factor | 0.19577 |
| Rwork | 0.193 |
| R-free | 0.24050 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.290 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.4.0062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.320 | 2.080 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.050 | 0.470 |
| Number of reflections | 66543 | |
| <I/σ(I)> | 9.1 | 1.5 |
| Completeness [%] | 82.7 | 65.9 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 320 MM AMMONIUM CITRATE, PH 5.6, 2% MPD, 15% W/V POLYETHYLENE GLYCOL 2000 MME |
