2W1W
Native structure of a family 35 carbohydrate binding module from Clostridium thermocellum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 46.386, 46.386, 204.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 204.120 - 1.550 |
| R-factor | 0.134 |
| Rwork | 0.132 |
| R-free | 0.17100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.525 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.100 | 0.200 |
| Number of reflections | 37854 | |
| <I/σ(I)> | 14.3 | 9 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 7.7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES, PH 7.5, 0.8 M SODIUM PHOSPHATE MONOBASIC, 0.8 M POTASSIUM PHOSPHATE MONOBASIC |
