2W1U
A family 32 carbohydrate-binding module, from the Mu toxin produced by Clostridium perfringens, in complex with beta-D-glcNAc-beta(1,3) galNAc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 113.15 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 91.170, 91.170, 132.607 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.17143 |
| Rwork | 0.169 |
| R-free | 0.21330 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.508 |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.890 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.100 |
| Number of reflections | 43009 |
| <I/σ(I)> | 10.4 |
| Completeness [%] | 98.6 |
| Redundancy | 6.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
