2W1S

Unique ligand binding specificity of a family 32 Carbohydrate-Binding Module from the Mu toxin produced by Clostridium perfringens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	113.15
Spacegroup name	P 21 21 21
Unit cell lengths	56.325, 61.847, 82.993
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.450
R-factor	0.176
Rwork	0.174
R-free	0.22600
Structure solution method	SIR
Starting model (for MR)	NONE
RMSD bond length	0.013
RMSD bond angle	1.460
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.740	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.060	0.380
Number of reflections	109314
<I/σ(I)>	15.7	3.2
Completeness [%]	99.1	91.6
Redundancy	7.08	4.16

Crystallization Conditions

crystal ID	method	pH	temperature	details
1